NBP1-31598

antibody from Novus Biologicals

Targeting: RPS3A MFTL, S3A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-31598

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-31598, RRID:AB_2238565

Product name

Rabbit Polyclonal RPS3A Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RPS3A Antibody [NBP1-31598] - Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE; antibody diluted at 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RPS3A Antibody [NBP1-31598] - Sample(30 ug whole cell lysate)A: 293T B: A431 C:H1299 D:HeLa S3 E: Hep G2 F: MOLT4 G: Raji 12% SDS PAGE, antibody diluted at 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RPS3A Antibody [NBP1-31598] - PS3A antibody detects RPS3A protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole cell lysate/extract. F. 30 ug Raw264.7 whole cell lysate/extract. G. 30 ug C2C12 whole cell lysate/extract. 12 % SDS-PAGE. RPS3A antibody dilution: 1:1000

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RPS3A Antibody [NBP1-31598] - RPS3A antibody detects RPS3A protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. B. 30 ug Rat2 whole cell lysate/extract. 12 % SDS-PAGE RPS3A antibody dilution: 1:1000.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RPS3A Antibody [NBP1-31598] - Paraffin-embedded U87 xenograft; antibody dilution 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RPS3A Antibody [NBP1-31598] - RPS3A antibody detects RPS3A protein at cytosol on mouse brain stem by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain stem. RPS3A antibody dilution: 1:200. Antigen Retrieval: Trilogy (EDTA based, pH 8.0) buffer, 15min