MAB19581

antibody from R&D Systems

Targeting: GDF11 BMP-11, BMP11

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB19581

Provider

R&D Systems

Product name

Human GDF-11/BMP-11 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human GDF-11/BMP-11 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human BMP-6 or recombinant mouse GDF-8 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

O95390


Isotype

IgG

Antibody clone number

743833

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

A GDF11/myostatin inhibitor, GDF11 propeptide-Fc, increases skeletal muscle mass and improves muscle strength in dystrophic mdx mice.

Jin Q, Qiao C, Li J, Xiao B, Li J, Xiao X
Skeletal muscle 2019 May 27;9(1):16

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Growth Differentiation Factor 11 treatment leads to neuronal and vascular improvements in the hippocampus of aged mice.

Ozek C, Krolewski RC, Buchanan SM, Rubin LL
Scientific reports 2018 Nov 23;8(1):17293

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Neonatal Systemic AAV-Mediated Gene Delivery of GDF11 Inhibits Skeletal Muscle Growth.

Jin Q, Qiao C, Li J, Li J, Xiao X
Molecular therapy : the journal of the American Society of Gene Therapy 2018 Apr 4;26(4):1109-1117

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GDF11 Attenuates Development of Type 2 Diabetes via Improvement of Islet β-Cell Function and Survival.

Li H, Li Y, Xiang L, Zhang J, Zhu B, Xiang L, Dong J, Liu M, Xiang G
Diabetes 2017 Jul;66(7):1914-1927

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Modulation of GDF11 expression and synaptic plasticity by age and training.

De Domenico E, D'Arcangelo G, Faraoni I, Palmieri M, Tancredi V, Graziani G, Grimaldi P, Tentori L
Oncotarget 2017 Aug 29;8(35):57991-58002

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Supraphysiological levels of GDF11 induce striated muscle atrophy.

Hammers DW, Merscham-Banda M, Hsiao JY, Engst S, Hartman JJ, Sweeney HL
EMBO molecular medicine 2017 Apr;9(4):531-544

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Activin A more prominently regulates muscle mass in primates than does GDF8.

Latres E, Mastaitis J, Fury W, Miloscio L, Trejos J, Pangilinan J, Okamoto H, Cavino K, Na E, Papatheodorou A, Willer T, Bai Y, Hae Kim J, Rafique A, Jaspers S, Stitt T, Murphy AJ, Yancopoulos GD, Gromada J
Nature communications 2017 Apr 28;8:15153

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

GDF-11/BMP-11 in Human Colon Cancer Tissue. GDF-11/BMP-11 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human GDF-11/BMP-11 Monoclonal Antibody (Catalog # MAB19581) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to epithelia of the colon. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.