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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- GTX89866 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX89866, RRID:AB_10731974
- Product name
- UBE2C antibody, C-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Goat
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western Blot of mock transfected (Lane 1) or UBE2C-transfected (Lane 2) U2OS cell lysate using GTX89866 at a 2.5 ?g/ml concentration. A band of approximately 20 kDa is detected.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GTX89866 detects a band of approximately 20 kDa after immmunoprecipitation from HeLa whole cell lysate (106 cells/ml) using 40 ?L of GTX23935 saturated protein G dynabeads.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GTX 89866 at a 1/200 dilution staining human UBE2C in paraformaldehyde fixed HeLa cells by Immunocytochemistry. In the upper panel, G2 phase cells can be seen (G1 cells stained only faintly). The lower panel shows a mitotic (metaphase) cell. Diffuse cytoplamic staining with some microtubule localization can be observed in mitotic cells.
