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- Antibody Data
- Antigen structure
- References [2]
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- Western blot [1]
- Flow cytometry [1]
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- Product number
- NB100-1084 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1084, RRID:AB_10000629
- Product name
- Goat Polyclonal UbcH10/UBE2C Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise all reported isoforms. Variants NP_861517.1 and NP_861518.1 encode the same isoform.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Dominant-negative cyclin-selective ubiquitin carrier protein E2-C/UbcH10 blocks cells in metaphase.
Dominant-negative cyclin-selective ubiquitin carrier protein E2-C/UbcH10 blocks cells in metaphase.
Townsley FM, Aristarkhov A, Beck S, Hershko A, Ruderman JV
Proceedings of the National Academy of Sciences of the United States of America 1997 Mar 18;94(6):2362-7
Proceedings of the National Academy of Sciences of the United States of America 1997 Mar 18;94(6):2362-7
Dominant-negative cyclin-selective ubiquitin carrier protein E2-C/UbcH10 blocks cells in metaphase.
Townsley FM, Aristarkhov A, Beck S, Hershko A, Ruderman JV
Proceedings of the National Academy of Sciences of the United States of America 1997 Mar 18;94(6):2362-7
Proceedings of the National Academy of Sciences of the United States of America 1997 Mar 18;94(6):2362-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UbcH10/UBE2C Antibody [NB100-1084] - (2ug/ml) staining of HEK293 (A) and HeLa (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: UbcH10/UBE2C Antibody [NB100-1084] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
