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LearnH00055294-B01P
antibody from Abnova Corporation
Targeting: FBXW7
AGO, CDC4, FBW7, FBX30, FBXW6, FLJ11071, SEL-10, SEL10
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
Reference
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- Product number
- H00055294-B01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00055294-B01P, RRID:AB_1573514
- Product name
- FBXW7 purified MaxPab mouse polyclonal antibody (B01P)
- Antibody type
- Polyclonal
- Description
- Mouse polyclonal antibody raised against a full-length human FBXW7 protein.
- Antigen sequence
MCVPRSGLILSCICLYCGVLLPVLLPNLPFLTCLS
MSTLESVTYLPEKGLYCQRLPSSRTHGGTESLKGK
NTENMGFYGTLKMIFYKMKRKLDHGSEVRSFSLGK
KPCKVSEYTSTTGLVPCSATPTTFGDLRAANGQGQ
QRRRITSVQPPTGLQEWLKMFQSWSGPEKLLALDE
LIDSCEPTQVKHMMQVIEPQFQRDFISLLPKELAL
YVLSFLEPKDLLQAAQTCRYWRILAEDNLLWREKC
KEEGIDEPLHIKRRKVIKPGFIHSPWKSAYIRQHR
IDTNWRRGELKSPKVLKGHDDHVITCLQFCGNRIV
SGSDDNTLKVWSAVTGKCLRTLVGHTGGVWSSQMR
DNIIISGSTDRTLKVWNAETGECIHTLYGHTSTVR
CMHLHEKRVVSGSRDATLRVWDIETGQCLHVLMGH
VAAVRCVQYDGRRVVSGAYDFMVKVWDPETETCLH
TLQGHTNRVYSLQFDGIHVVSGSLDTSIRVWDVET
GNCIHTLTGHQSLTSGMELKDNILVSGNADSTVKI
WDIKTGQCLQTLQGPNKHQSAVTCLQFNKNFVITS
SDDGTVKLWDLKTGEFIRNLVTLESGGSGGVVWRI
RASNTKLVCAVGSRNGTEETKLLVLDFDVDMK- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Cell cycle-dependent ubiquitylation and destruction of NDE1 by CDK5-FBW7 regulates ciliary length.
Maskey D, Marlin MC, Kim S, Kim S, Ong EC, Li G, Tsiokas L
The EMBO journal 2015 Oct 1;34(19):2424-40
The EMBO journal 2015 Oct 1;34(19):2424-40
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of FBXW7 expression in transfected 293T cell line (H00055294-T01) by FBXW7 MaxPab polyclonal antibody.Lane 1: FBXW7 transfected lysate(68.97 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to FBXW7 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
