MAB4609

antibody from R&D Systems

Targeting: CD151 PETA-3, RAPH, SFA-1, TSPAN24

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB4609

Provider

R&D Systems

Product name

Mouse CD151 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse CD151. Stains mouse CD151 transfectants but not irrelevant transfectants.

Reactivity

Mouse

Host

Rat

Conjugate

Unconjugated

Antigen sequence

BAA22447


Isotype

IgG

Antibody clone number

455807

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

CD151 in bEnd.3 Mouse Cell Line. CD151 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Rat Anti-Mouse CD151 Monoclonal Antibody (Catalog # MAB4609) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of CD151 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse CD151 Monoclonal Antibody (Catalog # MAB4609, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂ Secondary Antibody (Catalog # F0105B).