15938-1-AP

antibody from Invitrogen Antibodies

Targeting: CNOT10 FLJ12890, FLJ13165

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15938-1-AP

Provider

Invitrogen Antibodies

Product name

CNOT10 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: NPENVTDVS LGISSNEQDQ GSDKGENEAM ESSGKRAPQC YPSSVNSART VMLFNLGSAY CLRSEYDKAR KCLHQAASMI HPKEVPPEAI LLAVYLELQN GNTQLALQII KRNQLLPAVK THSEVRKKPV FQPVHPIQPI QMSAFTTVQR K (568-717 aa encoded by BC002928)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.23 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 15938-1-AP (CNOT10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human testis tissue were subjected to SDS PAGE followed by western blot with 15938-1-AP (CNOT10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using CNOT10 antibody 15938-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 15938-1-AP (CNOT10 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 15938-1-AP (CNOT10 antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-CNOT10 (IP:15938-1-AP, 4ug; Detection:15938-1-AP 1:300) with mouse brain tissue lysate 5200ug.