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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- H00001606-B01P - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001606-B01P, RRID:AB_2292782
- Product name
- Mouse Polyclonal DGK-alpha Antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified. DGKA - diacylglycerol kinase, alpha 80kDa,
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.05 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Diacylglycerol kinase-ζ regulates mTORC1 and lipogenic metabolism in cancer cells through SREBP-1.
Diacylglycerol kinase α promotes 3D cancer cell growth and limits drug sensitivity through functional interaction with Src.
Identification of candidate genes encoding an LDL-C QTL in baboons.
Diacylglycerol kinase α regulates the formation and polarisation of mature multivesicular bodies involved in the secretion of Fas ligand-containing exosomes in T lymphocytes.
Torres-Ayuso P, Tello-Lafoz M, Mérida I, Ávila-Flores A
Oncogenesis 2015 Aug 24;4:e164
Oncogenesis 2015 Aug 24;4:e164
Diacylglycerol kinase α promotes 3D cancer cell growth and limits drug sensitivity through functional interaction with Src.
Torres-Ayuso P, Daza-Martín M, Martín-Pérez J, Ávila-Flores A, Mérida I
Oncotarget 2014 Oct 30;5(20):9710-26
Oncotarget 2014 Oct 30;5(20):9710-26
Identification of candidate genes encoding an LDL-C QTL in baboons.
Karere GM, Glenn JP, Birnbaum S, Hafizi S, Rainwater DL, Mahaney MC, VandeBerg JL, Cox LA
Journal of lipid research 2013 Jul;54(7):1776-85
Journal of lipid research 2013 Jul;54(7):1776-85
Diacylglycerol kinase α regulates the formation and polarisation of mature multivesicular bodies involved in the secretion of Fas ligand-containing exosomes in T lymphocytes.
Alonso R, Mazzeo C, Rodriguez MC, Marsh M, Fraile-Ramos A, Calvo V, Avila-Flores A, Merida I, Izquierdo M
Cell death and differentiation 2011 Jul;18(7):1161-73
Cell death and differentiation 2011 Jul;18(7):1161-73
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DGK-alpha Antibody [H00001606-B01P] - Analysis of DGKA expression in transfected 293T cell line by DGKA polyclonal antibody. Lane 1: DGKA transfected lysate(80.85 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DGK-alpha Antibody [H00001606-B01P] - Analysis of DGKA expression in human spleen.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: DGK-alpha Antibody [H00001606-B01P] - Analysis of purified antibody to DGKA on HeLa cell. (antibody concentration 10 ug/ml)
