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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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- Product number
- SP5151P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#SP5151P, RRID:AB_1006860
- Product name
- anti RAGE
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to residues 362-380 of Rat RAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot detection of RAGE from Mouse lung extract using RAGE antibody SP5151P.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- SP5151P RAGE antibody Immunohistochemical staining of transgenic Mouse retinas.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RAGE in C6 Cells (left), HUVEC Cells (middle) and NIH-3T3 (right). Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RAGE polyclonal antibody (SP5151P) at a dilution of 1/200 overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. RAGE staining (green), F-Actin staining with Phalloidin (Red) and nuclei with DAPI (Bue) is shown. Images were taken at 60X magnification.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse lymph node tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/20 with RAGE Antibody Cat.-No SP5151P or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quencehd with a peroxidase suppressor. Detection was performed using Biotin conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse kidney tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/20 with RAGE Antibody Cat.-No SP5151P or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quencehd with a peroxidase suppressor. Detection was performed using Biotin conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse heart tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/20 with RAGE Antibody Cat.-No SP5151P or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quencehd with a peroxidase suppressor. Detection was performed using Biotin conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
