Explore
Validate
LearnNB100-57845
antibody from Novus Biologicals
Targeting: OGT
FLJ23071, HRNT1, MGC22921, O-GLCNAC, OGT1
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-57845 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-57845, RRID:AB_2157072
- Product name
- Goat Polyclonal O-GlcNAc Transferase p110 subunit Antibody
- Antibody type
- Polyclonal
Submitted references Insulin stimulates and diabetes inhibits O-linked N-acetylglucosamine transferase and O-glycosylation of Sp1.
Insulin stimulates and diabetes inhibits O-linked N-acetylglucosamine transferase and O-glycosylation of Sp1.
Majumdar G, Wright J, Markowitz P, Martinez-Hernandez A, Raghow R, Solomon SS
Diabetes 2004 Dec;53(12):3184-92
Diabetes 2004 Dec;53(12):3184-92
Insulin stimulates and diabetes inhibits O-linked N-acetylglucosamine transferase and O-glycosylation of Sp1.
Majumdar G, Wright J, Markowitz P, Martinez-Hernandez A, Raghow R, Solomon SS
Diabetes 2004 Dec;53(12):3184-92
Diabetes 2004 Dec;53(12):3184-92
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NB100-57845] - Rat pancreas lysate (35 ug protein in RIPA buffer). Primary antibody at 0.05 ug/mL, 1 hr incubation. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NB100-57845] - Human cortex tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: O-GlcNAc Transferase p110 subunit Antibody [NB100-57845] - Paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
