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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NB100-1212 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1212, RRID:AB_2157498
- Product name
- Goat Polyclonal NUP50 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise both reported isoforms (NP_009103.2
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Fast regulation of AP-1 activity through interaction of lamin A/C, ERK1/2, and c-Fos at the nuclear envelope.
Regulation of Wnt signaling by the nuclear pore complex.
Interaction of the melanocortin 2 receptor with nucleoporin 50: evidence for a novel pathway between a G-protein-coupled receptor and the nucleus.
A mechanism of AP-1 suppression through interaction of c-Fos with lamin A/C.
Npap60/Nup50 is a tri-stable switch that stimulates importin-alpha:beta-mediated nuclear protein import.
Npap60/Nup50 is a tri-stable switch that stimulates importin-alpha:beta-mediated nuclear protein import.
González JM, Navarro-Puche A, Casar B, Crespo P, Andrés V
The Journal of cell biology 2008 Nov 17;183(4):653-66
The Journal of cell biology 2008 Nov 17;183(4):653-66
Regulation of Wnt signaling by the nuclear pore complex.
Shitashige M, Satow R, Honda K, Ono M, Hirohashi S, Yamada T
Gastroenterology 2008 Jun;134(7):1961-71, 1971.e1-4
Gastroenterology 2008 Jun;134(7):1961-71, 1971.e1-4
Interaction of the melanocortin 2 receptor with nucleoporin 50: evidence for a novel pathway between a G-protein-coupled receptor and the nucleus.
Doufexis M, Storr HL, King PJ, Clark AJ
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2007 Dec;21(14):4095-100
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2007 Dec;21(14):4095-100
A mechanism of AP-1 suppression through interaction of c-Fos with lamin A/C.
Ivorra C, Kubicek M, González JM, Sanz-González SM, Alvarez-Barrientos A, O'Connor JE, Burke B, Andrés V
Genes & development 2006 Feb 1;20(3):307-20
Genes & development 2006 Feb 1;20(3):307-20
Npap60/Nup50 is a tri-stable switch that stimulates importin-alpha:beta-mediated nuclear protein import.
Lindsay ME, Plafker K, Smith AE, Clurman BE, Macara IG
Cell 2002 Aug 9;110(3):349-60
Cell 2002 Aug 9;110(3):349-60
Npap60/Nup50 is a tri-stable switch that stimulates importin-alpha:beta-mediated nuclear protein import.
Lindsay ME, Plafker K, Smith AE, Clurman BE, Macara IG
Cell 2002 Aug 9;110(3):349-60
Cell 2002 Aug 9;110(3):349-60
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NUP50 Antibody [NB100-1212] - Analysis of NUP50 in Jurkat lysate using NB100-1212 at 0.5 ug/ ml. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NUP50 Antibody [NB100-1212] - Staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NUP50 Antibody [NB100-1212] - 3.8ug/ml staining of Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
