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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [6]
- Flow cytometry [1]
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- Product number
- GTX84691 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX84691, RRID:AB_10727313
- Product name
- Chk2 antibody [5C4]
- Antibody type
- Monoclonal
- Reactivity
- Human, Canine
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CHEK2 monoclonal antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-CHEK2 mouse monoclonal antibody (GTX84691) immunofluorescent staining of COS7 cells transiently transfected with CHEK2
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-CHEK2 mouse monoclonal antibody (GTX84691).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded breast tissue using anti-CHEK2 mouse monoclonal antibody. (GTX84691, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded lung tissue using anti-CHEK2mouse monoclonal antibody. (GTX84691, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-CHEK2mouse monoclonal antibody. (GTX84691, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded colon tissue using anti-CHEK2mouse monoclonal antibody. (GTX84691, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-CHEK2mouse monoclonal antibody. (GTX84691, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded liver tissue using anti-CHEK2mouse monoclonal antibody. (GTX84691, Dilution 1:50)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-CHEK2 antibody (GTX84691), (Red), compared to a nonspecific negative control antibody, (Blue).
