Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 686602 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#686602, RRID:AB_2616885
- Product name
- Purified anti-Chk2
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant fragment of Chk2 (AA: 481-531) expressed in E.Coli.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- P81C12B8
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysate (15 µg protein) from 293T, Jurkat, HepG2 and NIH3T3 cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-CHK2 (clone P81C12B8) antibody. Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody and chemiluminescence detection.
- Conjugate
- Unconjugated