686602

antibody from BioLegend

Targeting: CHEK2 bA444G7, CDS1, CHK2, HuCds1, PP1425, RAD53

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

686602

Provider

BioLegend

Proper citation

BioLegend Cat#686602, RRID:AB_2616885

Product name

Purified anti-Chk2

Antibody type

Monoclonal

Antigen

Purified recombinant fragment of Chk2 (AA: 481-531) expressed in E.Coli.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

P81C12B8

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysate (15 µg protein) from 293T, Jurkat, HepG2 and NIH3T3 cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-CHK2 (clone P81C12B8) antibody. Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody and chemiluminescence detection.

Conjugate

Unconjugated