AF1626

antibody from R&D Systems

Targeting: CHEK2 bA444G7, CDS1, CHK2, HuCds1, PP1425, RAD53

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1626

Provider

R&D Systems

Product name

Human Phospho-Chk2 (T68) Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human Chk2 when phosphorylated at T68.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Starvation-induced activation of ATM/Chk2/p53 signaling sensitizes cancer cells to cisplatin.

Shi Y, Felley-Bosco E, Marti TM, Orlowski K, Pruschy M, Stahel RA
BMC cancer 2012 Dec 4;12:571

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Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen.

Boichuk S, Hu L, Hein J, Gjoerup OV
Journal of virology 2010 Aug;84(16):8007-20

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RAD51C facilitates checkpoint signaling by promoting CHK2 phosphorylation.

Badie S, Liao C, Thanasoula M, Barber P, Hill MA, Tarsounas M
The Journal of cell biology 2009 May 18;185(4):587-600

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Simian virus 40 large T antigen disrupts genome integrity and activates a DNA damage response via Bub1 binding.

Hein J, Boichuk S, Wu J, Cheng Y, Freire R, Jat PS, Roberts TM, Gjoerup OV
Journal of virology 2009 Jan;83(1):117-27

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Sonic Hedgehog signaling impairs ionizing radiation-induced checkpoint activation and induces genomic instability.

Leonard JM, Ye H, Wetmore C, Karnitz LM
The Journal of cell biology 2008 Nov 3;183(3):385-91

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RNF8-dependent and RNF8-independent regulation of 53BP1 in response to DNA damage.

Sakasai R, Tibbetts R
The Journal of biological chemistry 2008 May 16;283(20):13549-55

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Phospho-Chk2 (T68) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-Chk2 (T68) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1626) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-Chk2 (T68) was detected at approximately 64 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase ( lambda-PPase) for 60 minutes. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Phospho-Chk2 (T68) in HepG2 Human Cell Line. Phopsho-Chk2 (T68) was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line treated with 1 µM camptothecin using Rabbit Anti-Human Phospho-Chk2 (T68) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1626) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei in treated cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.