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Learn200-301-B07S
antibody from Rockland Immunochemicals, Inc.
Targeting: GDF15
MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 200-301-B07S - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-301-B07S, RRID:AB_10703687
- Product name
- Anti-NAG-1 (C-terminal specific) (MOUSE) Monoclonal Antibody - 200-301-B07S
- Antibody type
- Monoclonal
- Vial size
- 25 µl
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's anti-NAG-1 monoclonal antibody. The blot shows detection of recombinant NAG-1 protein present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag, lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag, lane 3 - yeast cell lysate expressing NAG-1 H variant and lane 4 - yeast cell lysate expressing NAG-1 D variant. Recombinant NAG-1 proteins correspond to 32 kDa and 16 kDa bands as indicated by the arrowheads. All lysates were run under reducing conditions. Primary antibody was used at a 1:1,000 dilution in TBS containg 1% BSA and 0.2% Tween, and reacted overnight at 4°C. Detection occurred using a 1:40,000 dilution of peroxidase conjugated Gt-a-Mouse IgG secondary antibody (610-103-121) in Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc™ 4000MP Imaging System. Other detection systems will yield similar results.
- Validation comment
- Western Blot
