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LearnPA5-120025
antibody from Invitrogen Antibodies
Targeting: GDF15
MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-120025 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GDF15 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive test controls include: PC-3, HT-1080. The target is usually found in the following locations: Secreted.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.82 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GDF15 in HT-1080 cells using a GDF15 Polyclonal antibody (Product # PA5-120025) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GDF15 in various cell lines using a GDF15 Polyclonal antibody (Product # PA5-120025) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 1s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of GDF15 in BALB-3T3 cells using a GDF15 Polyclonal antibody (Product # PA5-120025). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of GDF15 in BALB-3T3 cells using a GDF15 Polyclonal antibody (Product # PA5-120025). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GDF15 in human placenta using a Polyclonal antibody (Product # PA5-120025) at a dilution of 1:100. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol..
