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- References [8]
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- Western blot [1]
- Immunohistochemistry [7]
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- Product number
- NBP1-81050 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-81050, RRID:AB_11025173
- Product name
- Rabbit Polyclonal GDF-15 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human GDF-15 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Reconstitution of TGFBR2-Mediated Signaling Causes Upregulation of GDF-15 in HCT116 Colorectal Cancer Cells.
Association of serum level of growth differentiation factor 15 with liver cirrhosis and hepatocellular carcinoma.
The role of growth differentiation factor 15 in the pathogenesis of primary myelofibrosis.
GDF15 derived from both tumor-associated macrophages and esophageal squamous cell carcinomas contributes to tumor progression via Akt and Erk pathways.
GDF-15 is abundantly expressed in plexiform lesions in patients with pulmonary arterial hypertension and affects proliferation and apoptosis of pulmonary endothelial cells.
Growth differentiation factor 15: a prognostic marker for recurrence in colorectal cancer.
Primary ovarian mucinous carcinoma of intestinal type: significance of pattern of invasion and immunohistochemical expression profile in a series of 31 cases.
GDF-15 contributes to proliferation and immune escape of malignant gliomas.
Lee J, Fricke F, Warnken U, Schnölzer M, Kopitz J, Gebert J
PloS one 2015;10(6):e0131506
PloS one 2015;10(6):e0131506
Association of serum level of growth differentiation factor 15 with liver cirrhosis and hepatocellular carcinoma.
Liu X, Chi X, Gong Q, Gao L, Niu Y, Chi X, Cheng M, Si Y, Wang M, Zhong J, Niu J, Yang W
PloS one 2015;10(5):e0127518
PloS one 2015;10(5):e0127518
The role of growth differentiation factor 15 in the pathogenesis of primary myelofibrosis.
Uchiyama T, Kawabata H, Miura Y, Yoshioka S, Iwasa M, Yao H, Sakamoto S, Fujimoto M, Haga H, Kadowaki N, Maekawa T, Takaori-Kondo A
Cancer medicine 2015 Oct;4(10):1558-72
Cancer medicine 2015 Oct;4(10):1558-72
GDF15 derived from both tumor-associated macrophages and esophageal squamous cell carcinomas contributes to tumor progression via Akt and Erk pathways.
Urakawa N, Utsunomiya S, Nishio M, Shigeoka M, Takase N, Arai N, Kakeji Y, Koma Y, Yokozaki H
Laboratory investigation; a journal of technical methods and pathology 2015 May;95(5):491-503
Laboratory investigation; a journal of technical methods and pathology 2015 May;95(5):491-503
GDF-15 is abundantly expressed in plexiform lesions in patients with pulmonary arterial hypertension and affects proliferation and apoptosis of pulmonary endothelial cells.
Nickel N, Jonigk D, Kempf T, Bockmeyer CL, Maegel L, Rische J, Laenger F, Lehmann U, Sauer C, Greer M, Welte T, Hoeper MM, Golpon HA
Respiratory research 2011 May 6;12:62
Respiratory research 2011 May 6;12:62
Growth differentiation factor 15: a prognostic marker for recurrence in colorectal cancer.
Wallin U, Glimelius B, Jirström K, Darmanis S, Nong RY, Pontén F, Johansson C, Påhlman L, Birgisson H
British journal of cancer 2011 May 10;104(10):1619-27
British journal of cancer 2011 May 10;104(10):1619-27
Primary ovarian mucinous carcinoma of intestinal type: significance of pattern of invasion and immunohistochemical expression profile in a series of 31 cases.
Tabrizi AD, Kalloger SE, Köbel M, Cipollone J, Roskelley CD, Mehl E, Gilks CB
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 2010 Mar;29(2):99-107
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 2010 Mar;29(2):99-107
GDF-15 contributes to proliferation and immune escape of malignant gliomas.
Roth P, Junker M, Tritschler I, Mittelbronn M, Dombrowski Y, Breit SN, Tabatabai G, Wick W, Weller M, Wischhusen J
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 Aug 1;16(15):3851-9
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 Aug 1;16(15):3851-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GDF-15 Antibody [NBP1-81050] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GDF15 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GDF-15 Antibody [NBP1-81050] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GDF-15 Antibody [NBP1-81050] - Staining of human prostate shows moderate cytoplasmic positivity in a small subset of glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GDF-15 Antibody [NBP1-81050] - Staining of human placenta shows very strong cytoplasmic positivity in trophoblastic cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GDF-15 Antibody [NBP1-81050] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GDF-15 Antibody [NBP1-81050] - Analysis in human placenta and testis tissues. Corresponding GDF15 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GDF-15 Antibody [NBP1-81050] - Staining of human cervix shows no positivity in squamous epithelial cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GDF-15 Antibody [NBP1-81050] - Analysis in human placenta and cervix, uterine tissues. Corresponding GDF15 RNA-seq data are presented for the same tissues.
