Explore
Validate
LearnMAB1248-100
antibody from R&D Systems
Targeting: IL17B
IL-17B, IL-20, MGC138900, MGC138901, NIRF, ZCYTO7
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB1248-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-17B Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-17B in Western blots and ELISAs. In Western blots, approximately 25% cross-reactivity with recombinant mouse IL-17B is observed and no cross-reactivity with recombinant human (rh) IL-17, rhIL-17C, rhIL-17E or rhIL-17F is observed. In sandwich immunoassays, less than 0.5% cross-reactivity with rmIL-17B, and no cross-reactivity with rhIL-17C, rhIL-17D, rhIL-17E, rhIL-17F, or rhIL-17 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q9UHF5- Isotype
- IgG
- Antibody clone number
- 174113
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Autocrine/paracrine mechanism of interleukin-17B receptor promotes breast tumorigenesis through NF-κB-mediated antiapoptotic pathway.
Huang CK, Yang CY, Jeng YM, Chen CL, Wu HH, Chang YC, Ma C, Kuo WH, Chang KJ, Shew JY, Lee WH
Oncogene 2014 Jun 5;33(23):2968-77
Oncogene 2014 Jun 5;33(23):2968-77
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-17B in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Human IL-17B Monoclonal Antibody (Catalog # MAB1248, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
