Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- AF1709 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-17B Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse IL-17B in ELISAs and Western blots. In sandwich immunoassays, approximately 40% cross-reactivity with recombinant human IL-17B is observed and less than 0.3% cross-reactivity with recombinant mouse (rm) IL-17, rmIL-17C, rmIL-17D, rmIL-17E, and rmIL-17F is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9QXT6
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references IL-17B and IL-17C are associated with TNF-alpha production and contribute to the exacerbation of inflammatory arthritis.
IL-17B and IL-17C are associated with TNF-alpha production and contribute to the exacerbation of inflammatory arthritis.
Yamaguchi Y, Fujio K, Shoda H, Okamoto A, Tsuno NH, Takahashi K, Yamamoto K
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):7128-36
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):7128-36
IL-17B and IL-17C are associated with TNF-alpha production and contribute to the exacerbation of inflammatory arthritis.
Yamaguchi Y, Fujio K, Shoda H, Okamoto A, Tsuno NH, Takahashi K, Yamamoto K
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):7128-36
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):7128-36
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-17B in M-NFS-60 Mouse Cell Line by Flow Cytometry. M-NFS-60 mouse myelogenous leukemia lymphoblast cell line was stained with Goat Anti-Mouse IL-17B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1709, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.