200-301-A28

antibody from Rockland Immunochemicals, Inc.

Targeting: HSPA1A HSP70-1, HSPA1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

200-301-A28

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#200-301-A28, RRID:AB_2248558

Product name

Anti-Hsc70 (Hsp73) (MOUSE) Monoclonal Antibody - 200-301-A28

Antibody type

Monoclonal

Vial size

100 µl

References

Submitted references

Transcription Factor TonEBP Stimulates Hyperosmolality-Dependent Arginine Vasopressin Gene Expression in the Mouse Hypothalamus.

Kim DH, Kim KK, Lee TH, Eom H, Kim JW, Park JW, Jeong JK, Lee BJ
Frontiers in endocrinology 2021;12:627343

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TonEBP in dendritic cells mediates pro-inflammatory maturation and Th1/Th17 responses.

Ye BJ, Lee HH, Yoo EJ, Lee CY, Lee JH, Kang HJ, Jeong GW, Park H, Lee-Kwon W, Choi SY, Kwon HM
Cell death & disease 2020 Jun 4;11(6):421

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TonEBP/NFAT5 promotes obesity and insulin resistance by epigenetic suppression of white adipose tissue beiging.

Lee HH, An SM, Ye BJ, Lee JH, Yoo EJ, Jeong GW, Kang HJ, Alfadda AA, Lim SW, Park J, Lee-Kwon W, Kim JB, Choi SY, Kwon HM
Nature communications 2019 Aug 6;10(1):3536

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LPS-induced NFκB enhanceosome requires TonEBP/NFAT5 without DNA binding.

Lee HH, Sanada S, An SM, Ye BJ, Lee JH, Seo YK, Lee C, Lee-Kwon W, Küper C, Neuhofer W, Choi SY, Kwon HM
Scientific reports 2016 Apr 27;6:24921

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's Protein G purified anti-Hsc70 (Hsp73) antibody shows detection of Hsc70 (Hsp73) in whole cell lysates from heat shocked HeLa cells. The band marked by the double arrowheads corresponds to Hsc70 (Hsp73) at an approximate molecular weight of 72/73 kDa. The primary antibody was used at a 1:1,000 dilution.

Validation comment

Western Blot