ADI-SPA-820-F

antibody from Enzo Life Sciences

Targeting: HSPA1A HSP70-1, HSPA1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

ADI-SPA-820-F

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ADI-SPA-820-F, RRID:AB_11180104

Product name

HSC70/HSP70 monoclonal antibody (N27F3-4)

Antibody type

Monoclonal

Antigen

Purified from natural sources

Description

Protein G affinity purified.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Guinea Pig, Hamster, Porcine, Rabbit, Sheep, Simian, Xenopus

Host

Mouse

Isotype

IgG

Antibody clone number

N27F3-4

Vial size

200 μg

Storage

-20°C

References

Submitted references

Identification and characterization of a heat-inducible Hsp70 gene from Sorghum bicolor which confers tolerance to thermal stress.

Mulaudzi-Masuku T, Mutepe RD, Mukhoro OC, Faro A, Ndimba B
Cell stress & chaperones 2015 Sep;20(5):793-804

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Autophagy supports survival and phototransduction protein levels in rod photoreceptors.

Zhou Z, Doggett TA, Sene A, Apte RS, Ferguson TA
Cell death and differentiation 2015 Mar;22(3):488-98

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High levels of heat shock protein Hsp72 in cancer cells suppress default senescence pathways.

Yaglom JA, Gabai VL, Sherman MY
Cancer research 2007 Mar 1;67(5):2373-81

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Co-translational folding of caspase-activated DNase with Hsp70, Hsp40, and inhibitor of caspase-activated DNase.

Sakahira H, Nagata S
The Journal of biological chemistry 2002 Feb 1;277(5):3364-70

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Heat shock protein 70 moderately enhances peptide binding and transport by the transporter associated with antigen processing.

Chen D, Androlewicz MJ
Immunology letters 2001 Jan 1;75(2):143-8

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Heat shock protein 70 is able to prevent heat shock-induced resistance of target cells to CTL.

Dressel R, Elsner L, Quentin T, Walter L, Günther E
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 1;164(5):2362-71

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Hormone and antihormone induce distinct conformational changes which are central to steroid receptor activation.

Allan GF, Leng X, Tsai SY, Weigel NL, Edwards DP, Tsai MJ, O'Malley BW
The Journal of biological chemistry 1992 Sep 25;267(27):19513-20

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Binding of heat shock proteins to the avian progesterone receptor.

Kost SL, Smith DF, Sullivan WP, Welch WJ, Toft DO
Molecular and cellular biology 1989 Sep;9(9):3829-38

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Autoantibodies to the constitutive 73-kD member of the hsp70 family of heat shock proteins in systemic lupus erythematosus.

Minota S, Cameron B, Welch WJ, Winfield JB
The Journal of experimental medicine 1988 Oct 1;168(4):1475-80

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot analysis of HSC70/HSP70, mAb (N27F3-4) (Prod. No. ADI-SPA-820): Lane 1: MW marker; Lane 2: HSC70/HSP73 (bovine), (recombinant) (Prod. No. ADI-SPP-751); Lane 3: HSP70/HSP72 (human), (recombinant) (Prod. No. ADI-NSP-555); Lane 4: 3T3; Lane 5: 3T3 (heat shocked); Lane 6: HeLa; Lane 7: HeLa (heat shocked); Lane 8: PC-12; Lane 9: PC-12 (heat shocked).

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Immunohistochemistry analysis of human spleen tissue stained with HSC70/HSP70, mAb (N27F3-4) at 10µg/ml.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Flow cytometry analysis of human colon cancer Caco2 cells analyzed by flow cytometry using isotype control.