Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- NBP1-18880 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-18880, RRID:AB_1625599
- Product name
- Rabbit Polyclonal Casein Kinase 1 alpha Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references A Cytosolic Multiprotein Complex Containing p85α Is Required for β-Catenin Activation in Colitis and Colitis-associated Cancer.
Regulation of ribosomal protein S6 phosphorylation by casein kinase 1 and protein phosphatase 1.
Goretsky T, Bradford EM, Ryu H, Tahir M, Moyer MP, Gao T, Li L, Barrett TA
The Journal of biological chemistry 2016 Feb 19;291(8):4166-77
The Journal of biological chemistry 2016 Feb 19;291(8):4166-77
Regulation of ribosomal protein S6 phosphorylation by casein kinase 1 and protein phosphatase 1.
Hutchinson JA, Shanware NP, Chang H, Tibbetts RS
The Journal of biological chemistry 2011 Mar 11;286(10):8688-96
The Journal of biological chemistry 2011 Mar 11;286(10):8688-96
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Casein Kinase 1 alpha Antibody [NBP1-18880] - CKI alpha by Western Blot and Immunoprecipitation:CKI alpha Antibody [NBP1-18880]- Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-CKI alpha antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. CKI alpha was also immunoprecipitated another by rabbit anti-CKI alpha antibody which recognizes an upstream epitope.