H00006947-M12

antibody from Abnova Corporation

Targeting: TCN1 TC1, TCI

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006947-M12

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006947-M12, RRID:AB_1112177

Product name

TCN1 monoclonal antibody (M12), clone 3F10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant TCN1.

Antigen sequence

MRQSHQLPLVGLLLFSFIPSQLCEICEVSEENYIR
LKPLLNTMIQSNYNRGTSAVNVVLSLKLVGIQIQT
LMQKMIQQIKYNVKSRLSDVSSGELALIILALGVC
RNAEENLIYDYHLIDKLENKFQAEIENMEAHNGTP
LTNYYQLSLDVLALCLFNGNYSTAEVVNHFTPENK
NYYFGSQFSVDTGAMAVLALTCVKKSLINGQIKAD
EGSLKNISIYTKSLVEKILSEKKENGLIGNTFSTG
EAMQALFVSSDYYNENDWNCRQTLNTVLTEISQGA
FSNPNAAAQVLPALMGKTFLDINKDSSCVPASGNF
NISADEPITVTPPDSQSYISVDYSVRINETYFTNV
AVLNGSVFLSVMEKAQKMNDTIFGFTMEERSWGPY
ITCIQGLCANNNDRTYWELLSGGEPLSQGAGSYVV
RNG


Isotype

IgG

Antibody clone number

3F10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of TCN1 expression in transfected 293T cell line by TCN1 monoclonal antibody (M12), clone 3F10.Lane 1: TCN1 transfected lysate(48.2 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged TCN1 is 0.1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of TCN1 transfected lysate using anti-TCN1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with TCN1 MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to TCN1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol