H00010235-M09

antibody from Abnova Corporation

Targeting: RASGRP2 CALDAG-GEFI

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00010235-M09

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00010235-M09, RRID:AB_606917

Product name

RASGRP2 monoclonal antibody (M09), clone 3D8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant RASGRP2.

Antigen sequence

GTLDLDKGCTVEELLRGCIEAFDDSGKVRDPQLVR
MFLMMHPWYIPSSQLAAKLLHIYQQSRKDNSNSLQ
VKTCHLVRYWISAFPAEFDLNPELAEQIKE


Isotype

IgG

Antibody clone number

3D8

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Calcium-diacylglycerol guanine nucleotide exchange factor I gene mutations associated with loss of function in canine platelets.

Boudreaux MK, Catalfamo JL, Klok M
Translational research : the journal of laboratory and clinical medicine 2007 Aug;150(2):81-92

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of RASGRP2 expression in transfected 293T cell line by RASGRP2 monoclonal antibody (M09), clone 3D8.Lane 1: RASGRP2 transfected lysate(75.5 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged RASGRP2 is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol