- GTX129153 - GeneTex EHMT2 antibody

Submitted references Preprogramming therapeutic response of PI3K/mTOR dual inhibitor via the regulation of EHMT2 and p27 in pancreatic cancer.

Tian YF, Wang HC, Luo CW, Hung WC, Lin YH, Chen TY, Li CF, Lin CY, Pan MR
American journal of cancer research 2018;8(9):1812-1822

Targeting histone methyltransferase G9a inhibits growth and Wnt signaling pathway by epigenetically regulating HP1α and APC2 gene expression in non-small cell lung cancer.

Zhang K, Wang J, Yang L, Yuan YC, Tong TR, Wu J, Yun X, Bonner M, Pangeni R, Liu Z, Yuchi T, Kim JY, Raz DJ
Molecular cancer 2018 Oct 22;17(1):153

G9a governs colon cancer stem cell phenotype and chemoradioresistance through PP2A-RPA axis-mediated DNA damage response.

Luo CW, Wang JY, Hung WC, Peng G, Tsai YL, Chang TM, Chai CY, Lin CH, Pan MR
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology 2017 Sep;124(3):395-402

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Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody (GTX129153) diluted at 1:2000.
KD Reagent Type: siRNA
Downregulation: >50%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
Antibody Lot Number: 41570

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: G9a antibody detects G9a protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extract B. 30 £gg A431 whole cell lysate/extract C. 30 £gg HeLa whole cell lysate/extract |D. 30 £gg HepG2 whole cell lysate/extract5 % SDS-PAGEG9a antibody (GTX129153) dilution: 1:1000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: G9a antibody detects G9a protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody (GTX129153) diluted at 1:3000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody (GTX129153) diluted at 1:2000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: G9a antibody detects G9a protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: G9a protein stained by G9a antibody (GTX129153) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: G9a antibody immunoprecipitates G9a protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : Control with 3 £gg of pre-immune rabbit IgG B : Immunoprecipitation of G9a by 3 £gg of G9a antibody (GTX129153) 5% SDS-PAGE The immunoprecipitated G9a protein was detected by G9a antibody (GTX129153) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: G9a antibody detects G9a protein at nucleus on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. G9a antibody (GTX129153) diluted at 1:250.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ChIP was performed with HeLa chromatin extract and 5 ?g of either normal rabbit IgG or anti-G9a antibody. The precipitated DNA was detected by PCR with primer set targeting to FXN.

GTX129153

Antibody data