APR00334G

antibody from Leading Biology

Targeting: EHMT2 BAT8, C6orf30, Em:AF134726.3, G9A, KMT1C, NG36/G9a

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

APR00334G

Provider

Leading Biology

Product name

EHMT2 Antibody

Antibody type

Polyclonal

Description

G9a, also known as Euchromatic histone­lysine N­methyltransferase 2 (EHMT2), is a member of a family of histone lysine methyltransferases. Recombinant G9a can mono­, di­ and tri­methylate histone H3 on Lys9 and Lys27 in vitro. However, in vivo G9a forms a complex with GLP, a G9a­related histone methyltransferase, and together these proteins function as the major euchromatic histone H3 Lys9 mono­ and di­methyltransferases, creating transcriptionally repressive marks that facilitate gene silencing. G9a methylates itself on Lys165, a modification that regulates the association of HP1 repressor proteins with the G9a/GLP complex. The G9a/GLP complex also contains Wiz, a zinc finger protein that is required for G9a/GLP hetero­dimerization and complex stability. Wiz contains two CtBP co­repressor binding sites, which mediate the association of the G9a/GLP with the CtBP co­repressor complex. In addition, G9a and GLP are components of other large transcriptional co­repressor complexes, such as those involving E2F6 and CDP/cut. G9a interacts with DNMT1, and both proteins are required for methylation of DNA and histone H3 (Lys9) at replication foci, providing a functional link between histone H3 Lys9 and CpG methylation during DNA replication. G9a activity is critical for meiotic prophase progression, as mutant mice deficient in germ line G9a show a large loss of mature gametes. In addition, G9a facilitates increased global levels of di­methyl histone H3 (Lys9) during hypoxic stress and increased G9a expression is associated with hepatocellular carcinoma.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Storage

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.