PA5-28140

antibody from Invitrogen Antibodies

Targeting: FBXL2 FBL2, FBL3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28140

Provider

Invitrogen Antibodies

Product name

FBXL2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: A431, HeLa, HepG2, Raji.

Concentration

1.16 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FBXL2 using 30 µg of A) HeLa and B) Raji lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a FBXL2 polyclonal antibody (Product # PA5-28140) at a dilution of 1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using FBXL2 Polyclonal Antibody (Product # PA5-28140). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with FBXL2 Polyclonal Antibody (Product # PA5-28140) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of FBXL2 in methanol-fixed HeLa cells using a FBXL2 polyclonal antibody (Product # PA5-28140) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FBXL2 Polyclonal Antibody detects FBXL2 protein at nucleolus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: FBXL2 stained by FBXL2 Polyclonal Antibody (Product # PA5-28140) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Fluoroshield with DAPI .

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using FBXL2 (Product # PA5-28140) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.