PAB29326

antibody from Abnova Corporation

Targeting: EIF4ENIF1 2610509L04Rik, 4E-T, Clast4, FLJ21601

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29326

Provider

Abnova Corporation

Product name

EIF4ENIF1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human EIF4ENIF1.

Antigen sequence

SPLEKDSDGLRLLGGRRIGSGRIISARTFEKDHRL
SDKDLRDLRDRDRERDFKDKRFRREFGDSKRVFGE
RRRNDSYTEEEPEWFSAGPTSQSETIELTGFDDKI
LEEDHKGRKRTRRRTASVKEGIVECNGGVAEEDEV
E


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Human cell line RT-4; Lane 2: Human cell line U-251MG sp; Lane 3: Human plasma (IgG/HSA depleted) with EIF4ENIF1 polyclonal antibody (Cat# PAB29326) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with EIF4ENIF1 polyclonal antibody (Cat# PAB29326) under 1-4 ug/mL working concentration shows positivity in vesicles.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas with EIF4ENIF1 polyclonal antibody (Cat# PAB29326) shows strong cytoplasmic positivity in exocrine glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)