Explore
Validate
LearnH00056478-B01P
antibody from Abnova Corporation
Targeting: EIF4ENIF1
2610509L04Rik, 4E-T, Clast4, FLJ21601
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00056478-B01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00056478-B01P, RRID:AB_1673784
- Product name
- EIF4ENIF1 purified MaxPab mouse polyclonal antibody (B01P)
- Antibody type
- Polyclonal
- Description
- Mouse polyclonal antibody raised against a full-length human EIF4ENIF1 protein.
- Antigen sequence
MDRRSMGETESGDAFLDLKKPPASKCPHRYTKEEL
LDIKELPHSKQRPSCLSEKYDSDGVWDPEKWHASL
YPASGRSSPVESLKKELDTDRPSLVRRIVDPRERV
KEDDLDVVLSPQRRSFGGGCHVTAAVSSRRSGSPL
EKDSDGLRLLGGRRIGSGRIISARTFEKDHRLSDK
DLRDLRDRDRERDFKDKRFRREFGDSKRVFGERRR
NDSYTEEEPEWFSAGPTSQSETIELTGFDDKILEE
DHKGRKRTRRRTASVKEGIVECNGGVAEEDEVEVI
LAQEPAADQEVPRDAVLPEQSPGDFDFNEFFNLDK
VPCLASMIEDVLGEGSVSASRFSRWFSNPSRSGSR
SSSLGSTPHEELERLAGLEQAILSPGQNSGNYFAP
IPLEDHAENKVDILEMLQKAKVDLKPLLSSLSANK
EKLKESSHSGVVLSVEEVEAGLKGLKVDQQVKNST
PFMAEHLEETLSAVTNNRQLKKDGDMTAFNKLVST
MKASGTLPSQPKVSRNLESHLMSPAEIPGQPVPKN
ILQELLGQPVQRPASSNLLSGLMGSLEPTTSLLGQ
RAPSPPLSQVFQTRAASADYLRPRIPSPIGFTPGP
QQLLGDPFQGMRKPMSPITAQMSQLELQQAALEGL
ALPHDLAVQAANFYQPGFGKPQVDRTRDGFRNRQQ
RVTKSPAPVHRGNSSSPAPAASITSMLSPSFTPTS
VIRKMYESKEKSKEEPASGKAALGDSKEDTQKASE
ENLLSSSSVPSADRDSSPTTNSKLSALQRSSCSTP
LSQANRYTKEQDYRPKATGRKTPTLASPVPTTPFL
RPVHQVPLVPHVPMVRPAHRLHPGLVQRMLAQGVH
PQHLPSLLQTGVLPPGMDLSHLQGISGPILGQPFY
PLPAASHPLLNPRPGTPLHLAMVQQQLQRSVLHPP
GSGSHAAAVSVQTTPQNVPSRSGLPHMHSQLEHRP
SQRSSSPVGLAKWFGSDVLQQPLPSMPAKVISVDE
LEYRQ- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references A Smaug2-Based Translational Repression Complex Determines the Balance between Precursor Maintenance versus Differentiation during Mammalian Neurogenesis.
An eIF4E1/4E-T complex determines the genesis of neurons from precursors by translationally repressing a proneurogenic transcription program.
Amadei G, Zander MA, Yang G, Dumelie JG, Vessey JP, Lipshitz HD, Smibert CA, Kaplan DR, Miller FD
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Nov 25;35(47):15666-81
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Nov 25;35(47):15666-81
An eIF4E1/4E-T complex determines the genesis of neurons from precursors by translationally repressing a proneurogenic transcription program.
Yang G, Smibert CA, Kaplan DR, Miller FD
Neuron 2014 Nov 19;84(4):723-39
Neuron 2014 Nov 19;84(4):723-39
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of EIF4ENIF1 expression in transfected 293T cell line (H00056478-T02) by EIF4ENIF1 MaxPab polyclonal antibody.Lane 1: EIF4ENIF1 transfected lysate(108.35 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- EIF4ENIF1 MaxPab polyclonal antibody. Western Blot analysis of EIF4ENIF1 expression in Jurkat.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- EIF4ENIF1 MaxPab polyclonal antibody. Western Blot analysis of EIF4ENIF1 expression in Hela S3 NE.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to EIF4ENIF1 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
