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LearnNB100-1368
antibody from Novus Biologicals
Targeting: EIF4ENIF1
2610509L04Rik, 4E-T, Clast4, FLJ21601
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-1368 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1368, RRID:AB_2095741
- Product name
- Goat Polyclonal eIF4ENIF1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise isoform 1 (NP_062817.2) and isoform b (NP_001157974.1). Reported variants represent identical protein (NP_062817.2
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references A novel shuttling protein, 4E-T, mediates the nuclear import of the mRNA 5' cap-binding protein, eIF4E.
Dostie J, Ferraiuolo M, Pause A, Adam SA, Sonenberg N
The EMBO journal 2000 Jun 15;19(12):3142-56
The EMBO journal 2000 Jun 15;19(12):3142-56
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: eIF4ENIF1 Antibody [NB100-1368] - Analysis of eIF4ENIF1 (0.25ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: eIF4ENIF1 Antibody [NB100-1368] - IHC analysis of eIF4ENIF1 in paraffin embedded Human Pancreas using this antibody at 5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
