A300-797A-T

antibody from Invitrogen Antibodies

Targeting: ZNF335 bA465L10.2, NIF-1

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-797A-T

Provider

Invitrogen Antibodies

Product name

NIF1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse NIF1 by immunohistochemistry. Sample: FFPE section of human thyroid carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-NIF1 (Cat. No. A300-797A Lot1) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human NIF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NIF1 antibody BL2737 (Cat. No. A300-797A) used IP at 3 µg/mg lysate. NIF1 was also immunoprecipitated by rabbit anti-NIF1 antibodies BL2738 and BL2739 (Cat. No. A300-798A). For blotting immunoprecipitated NIF1, BL2739 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.