NB100-2578

antibody from Novus Biologicals

Targeting: ZNF335 bA465L10.2, NIF-1

Western blot (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-2578

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-2578, RRID:AB_2217445

Product name

Rabbit Polyclonal NIF1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: NIF1 Antibody [NB100-2578] - Detection of Human NIF1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NIF1 antibody NB100-2578 used IP at 3 mcg/mg lysate. NIF1 was also immunoprecipitated by rabbit anti-NIF1 antibodies NB100-2579 and another NIF1 Ab. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: NIF1 Antibody [NB100-2578] - Sample: FFPE section of human thyroid carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-NIF1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB