20770-1-AP

antibody from Proteintech Group

Targeting: GSDMD DF5L, FLJ12150, GSDMDC1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

20770-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#20770-1-AP, RRID:AB_10696319

Product name

GSDMD antibody

Antibody type

Polyclonal

Description

KD/KO validated GSDMD antibody (Cat. #20770-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: FC, IF, WB,ELISA.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Escin Sodium Improves the Prognosis of Acute Pancreatitis via Promoting Cell Apoptosis by Suppression of the ERK/STAT3 Signaling Pathway.

Zhang Q, Zhao C, Zhang L, Sun K, Yu L, Wang X, Ren L, Zhang N, Chen C, Liu J, Wang H, Tian H
Oxidative medicine and cellular longevity 2021;2021:9921839

---

Sodium Houttuyfonate Ameliorates β-amyloid_1-42-Induced Memory Impairment and Neuroinflammation through Inhibiting the NLRP3/GSDMD Pathway in Alzheimer's Disease.

Zhao Y, Tian Y, Feng T
Mediators of inflammation 2021;2021:8817698

---

Autophagic Degradation of Gasdermin D Protects against Nucleus Pulposus Cell Pyroptosis and Retards Intervertebral Disc Degeneration In Vivo.

Liao Z, Li S, Liu R, Feng X, Shi Y, Wang K, Li S, Zhang Y, Wu X, Yang C
Oxidative medicine and cellular longevity 2021;2021:5584447

---

Destructive Effects of Pyroptosis on Homeostasis of Neuron Survival Associated with the Dysfunctional BBB-Glymphatic System and Amyloid-Beta Accumulation after Cerebral Ischemia/Reperfusion in Rats.

Lyu Z, Chan Y, Li Q, Zhang Q, Liu K, Xiang J, Li X, Cai D, Li Y, Wang B, Yu Z
Neural plasticity 2021;2021:4504363

---

Metformin Corrects Glucose Metabolism Reprogramming and NLRP3 Inflammasome-Induced Pyroptosis via Inhibiting the TLR4/NF-κB/PFKFB3 Signaling in Trophoblasts: Implication for a Potential Therapy of Preeclampsia.

Zhang Y, Liu W, Zhong Y, Li Q, Wu M, Yang L, Liu X, Zou L
Oxidative medicine and cellular longevity 2021;2021:1806344

---

Cisplatin Induces Pyroptosis via Activation of MEG3/NLRP3/caspase-1/GSDMD Pathway in Triple-Negative Breast Cancer.

Yan H, Luo B, Wu X, Guan F, Yu X, Zhao L, Ke X, Wu J, Yuan J
International journal of biological sciences 2021;17(10):2606-2621

---

Assessment of Pyroptosis-Related Indicators as Potential Biomarkers and Their Association with Severity in Patients with Liver Cirrhosis.

Wang D, Zhan X, Wu R, You Y, Chen W, Duan L
Journal of inflammation research 2021;14:3185-3196

---

Metallothionein 3-Zinc Axis Suppresses Caspase-11 Inflammasome Activation and Impairs Antibacterial Immunity.

Chowdhury D, Gardner JC, Satpati A, Nookala S, Mukundan S, Porollo A, Landero Figueroa JA, Subramanian Vignesh K
Frontiers in immunology 2021;12:755961

---

CBD Alleviates Liver Injuries in Alcoholics With High-Fat High-Cholesterol Diet Through Regulating NLRP3 Inflammasome-Pyroptosis Pathway.

Jiang X, Gu Y, Huang Y, Zhou Y, Pang N, Luo J, Tang Z, Zhang Z, Yang L
Frontiers in pharmacology 2021;12:724747

---

siRNAs Targeting Mouse-Specific lncRNA AA388235 Induce Human Tumor Cell Pyroptosis/Apoptosis.

Chen YR, Feng WY, Cheng YX, Zhu H, Liu HJ, Gao Y, Zhou WJ
Frontiers in oncology 2021;11:662444

---

Injectable exosome-functionalized extracellular matrix hydrogel for metabolism balance and pyroptosis regulation in intervertebral disc degeneration.

Xing H, Zhang Z, Mao Q, Wang C, Zhou Y, Zhou X, Ying L, Xu H, Hu S, Zhang N
Journal of nanobiotechnology 2021 Sep 6;19(1):264

---

Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.

Liao Z, Liu H, Ma L, Lei J, Tong B, Li G, Ke W, Wang K, Feng X, Hua W, Li S, Yang C
ACS nano 2021 Sep 28;15(9):14709-14724

---

Plasma exosomes derived from patients with intestinal Behçet's syndrome induce intestinal epithelial cell pyroptosis.

Hou CC, Ma HF, Ye JF, Luo D, Bao HF, Guan JL
Clinical rheumatology 2021 Oct;40(10):4143-4155

---

Aronia melanocarpa polysaccharide ameliorates inflammation and aging in mice by modulating the AMPK/SIRT1/NF-κB signaling pathway and gut microbiota.

Zhao Y, Liu X, Zheng Y, Liu W, Ding C
Scientific reports 2021 Oct 18;11(1):20558

---

(-)-Epicatechin ameliorates cigarette smoke-induced lung inflammation via inhibiting ROS/NLRP3 inflammasome pathway in rats with COPD.

Tian X, Xue Y, Xie G, Zhou Y, Xiao H, Ding F, Zhang M
Toxicology and applied pharmacology 2021 Oct 15;429:115674

---

Crosstalk Between RPE Cells and Choroidal Endothelial Cells via the ANXA1/FPR2/SHP2/NLRP3 Inflammasome/Pyroptosis Axis Promotes Choroidal Neovascularization.

Zhu M, Wang Y, Zhu L, Du S, Wang Z, Zhang Y, Guo Y, Tu Y, Song E
Inflammation 2021 Oct 1;

---

The relationship between liver pathological inflammation degree and pyroptosis in chronic hepatitis B patients.

Wang Y, Li X, Chen Q, Jiao F, Shi C, Pei M, Wang L, Gong Z
Journal of medical virology 2021 Nov;93(11):6229-6235

---

Aluminum impairs cognitive function by activating DDX3X-NLRP3-mediated pyroptosis signaling pathway.

Hao W, Hao C, Wu C, Xu Y, Wu S, Lu X, Yang J, Jin C
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2021 Nov;157:112591

---

The balance between AIM2-associated inflammation and autophagy: the role of CHMP2A in brain injury after cardiac arrest.

Shao R, Wang X, Xu T, Xia Y, Cui D
Journal of neuroinflammation 2021 Nov 5;18(1):257

---

Gasdermin D Is a Novel Prognostic Biomarker and Relates to TMZ Response in Glioblastoma.

Liu J, Gao L, Zhu X, Geng R, Tao X, Xu H, Chen Z
Cancers 2021 Nov 10;13(22)

---

Mesenchymal stromal cells protect hepatocytes from lipotoxicity through alleviation of endoplasmic reticulum stress by restoring SERCA activity.

Li L, Zeng X, Liu Z, Chen X, Li L, Luo R, Liu X, Zhang J, Liu J, Lu Y, Cheng J, Chen Y
Journal of cellular and molecular medicine 2021 Mar;25(6):2976-2993

---

Gasdermin D mediates the maturation and release of IL-1α downstream of inflammasomes.

Tsuchiya K, Hosojima S, Hara H, Kushiyama H, Mahib MR, Kinoshita T, Suda T
Cell reports 2021 Mar 23;34(12):108887

---

Macrophage membrane-mediated targeted drug delivery for treatment of spinal cord injury regardless of the macrophage polarization states.

Tang W, Yang Y, Yang L, Tang M, Chen Y, Li C
Asian journal of pharmaceutical sciences 2021 Jul;16(4):459-470

---

Suppressing ROS generation by apocynin inhibited cyclic stretch-induced inflammatory reaction in HPDLCs via a caspase-1 dependent pathway.

Wang Y, Song Y, Zhong Q, Wu Y, Zhuang J, Qu F, Xu C
International immunopharmacology 2021 Jan;90:107129

---

Low shear stress induced vascular endothelial cell pyroptosis by TET2/SDHB/ROS pathway.

Chen J, Zhang J, Wu J, Zhang S, Liang Y, Zhou B, Wu P, Wei D
Free radical biology & medicine 2021 Jan;162:582-591

---

Histone deacetylase 2 regulates ULK1 mediated pyroptosis during acute liver failure by the K68 acetylation site.

Wang Y, Chen Q, Jiao F, Shi C, Pei M, Wang L, Gong Z
Cell death & disease 2021 Jan 11;12(1):55

---

Macrophages regulates the transition of pericyte to peritoneal fibrosis through the GSDMD/IL-1β axis.

Shao Q, Sun C, Zhang Q, Liu J, Xia Y, Jin B, Qian X
International immunopharmacology 2021 Dec;101(Pt A):108323

---

Isoorientin Attenuated the Pyroptotic Hepatocyte Damage Induced by Benzo[a]pyrene via ROS/NF-κB/NLRP3/Caspase-1 Signaling Pathway.

Li H, Yuan L, Li X, Luo Y, Zhang Z, Li J
Antioxidants (Basel, Switzerland) 2021 Aug 11;10(8)

---

Aspergillus fumigatus-Stimulated Human Corneal Epithelial Cells Induce Pyroptosis of THP-1 Macrophages by Secreting TSLP.

Ji Q, Wang L, Liu J, Wu Y, Lv H, Wen Y, Shi L, Qu B, Szentmáry N
Inflammation 2021 Apr;44(2):682-692

---

CD147 Expression Is Associated with Tumor Proliferation in Bladder Cancer via GSDMD.

Peng J, Jiang H, Guo J, Huang J, Yuan Q, Xie J, Xiao K
BioMed research international 2020;2020:7638975

---

Oleic acid ameliorates palmitic acid induced hepatocellular lipotoxicity by inhibition of ER stress and pyroptosis.

Zeng X, Zhu M, Liu X, Chen X, Yuan Y, Li L, Liu J, Lu Y, Cheng J, Chen Y
Nutrition & metabolism 2020;17:11

---

Overexpression of gasdermin D promotes invasion of adenoid cystic carcinoma.

Shen X, Zhang Q, He Z, Xiao S, Li H, Huang Z
International journal of clinical and experimental pathology 2020;13(7):1802-1811

---

Detection of proteins associated with the pyroptosis signaling pathway in breast cancer tissues and their significance.

Wu X, Mao X, Huang Y, Zhu Q, Guan J, Wu L
International journal of clinical and experimental pathology 2020;13(6):1408-1414

---

Tangshen Formula Attenuates Diabetic Kidney Injury by Imparting Anti-pyroptotic Effects via the TXNIP-NLRP3-GSDMD Axis.

Li N, Zhao T, Cao Y, Zhang H, Peng L, Wang Y, Zhou X, Wang Q, Li J, Yan M, Dong X, Zhao H, Li P
Frontiers in pharmacology 2020;11:623489

---

IFN regulatory Factor-1 induced macrophage pyroptosis by modulating m6A modification of circ_0029589 in patients with acute coronary syndrome.

Guo M, Yan R, Ji Q, Yao H, Sun M, Duan L, Xue Z, Jia Y
International immunopharmacology 2020 Sep;86:106800

---

Trimethylamine N-oxide promotes apoE^-/- mice atherosclerosis by inducing vascular endothelial cell pyroptosis via the SDHB/ROS pathway.

Wu P, Chen J, Chen J, Tao J, Wu S, Xu G, Wang Z, Wei D, Yin W
Journal of cellular physiology 2020 Oct;235(10):6582-6591

---

Type I Interferon Increases Inflammasomes Associated Pyroptosis in the Salivary Glands of Patients with Primary Sjögren's Syndrome.

Hong SM, Lee J, Jang SG, Lee J, Cho ML, Kwok SK, Park SH
Immune network 2020 Oct;20(5):e39

---

MiR-124-3p promotes trophoblast cell HTR-8/SVneo pyroptosis by targeting placental growth factor.

Tao J, Xia LZ, Liang L, Chen Y, Wei D, Meng J, Wu S, Wang Z
Placenta 2020 Nov;101:176-184

---

Exposure to hyperandrogen drives ovarian dysfunction and fibrosis by activating the NLRP3 inflammasome in mice.

Wang D, Weng Y, Zhang Y, Wang R, Wang T, Zhou J, Shen S, Wang H, Wang Y
The Science of the total environment 2020 Nov 25;745:141049

---

HMGB1 mediates homocysteine-induced endothelial cells pyroptosis via cathepsin V-dependent pathway.

Leng Y, Chen R, Chen R, He S, Shi X, Zhou X, Zhang Z, Chen AF
Biochemical and biophysical research communications 2020 Nov 19;532(4):640-646

---

NLRP3-dependent pyroptosis is required for HIV-1 gp120-induced neuropathology.

He X, Yang W, Zeng Z, Wei Y, Gao J, Zhang B, Li L, Liu L, Wan Y, Zeng Q, Gong Z, Liu L, Zhang H, Li Y, Yang S, Hu T, Wu L, Masliah E, Huang S, Cao H
Cellular & molecular immunology 2020 Mar;17(3):283-299

---

TNF-α/HMGB1 inflammation signalling pathway regulates pyroptosis during liver failure and acute kidney injury.

Wang Y, Zhang H, Chen Q, Jiao F, Shi C, Pei M, Lv J, Zhang H, Wang L, Gong Z
Cell proliferation 2020 Jun;53(6):e12829

---

Discovery of novel TNNI3K inhibitor suppresses pyroptosis and apoptosis in murine myocardial infarction injury.

Pang H, Wang N, Chai J, Wang X, Zhang Y, Bi Z, Wu W, He G
European journal of medicinal chemistry 2020 Jul 1;197:112314

---

Polyphyllin VI Induces Caspase-1-Mediated Pyroptosis via the Induction of ROS/NF-κB/NLRP3/GSDMD Signal Axis in Non-Small Cell Lung Cancer.

Teng JF, Mei QB, Zhou XG, Tang Y, Xiong R, Qiu WQ, Pan R, Law BY, Wong VK, Yu CL, Long HA, Xiao XL, Zhang F, Wu JM, Qin DL, Wu AG
Cancers 2020 Jan 13;12(1)

---

Cyclin-Dependent Kinase 9 Inhibition Suppresses Necroptosis and Pyroptosis in the Progress of Endotoxemia.

Li J, Mao H, Pan Y, Li H, Lei L
Inflammation 2020 Dec;43(6):2061-2074

---

Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis.

Zhang X, Zhang P, An L, Sun N, Peng L, Tang W, Ma D, Chen J
Acta pharmaceutica Sinica. B 2020 Aug;10(8):1397-1413

---

IFN Regulatory Factor 1 Mediates Macrophage Pyroptosis Induced by Oxidized Low-Density Lipoprotein in Patients with Acute Coronary Syndrome.

Guo M, Yan R, Yao H, Duan L, Sun M, Xue Z, Jia Y
Mediators of inflammation 2019;2019:2917128

---

HMGB1-induced endothelial cell pyroptosis is involved in systemic inflammatory response syndrome following radiofrequency ablation of hepatic hemangiomas.

Yang M, Yang X, Wang S, Xu L, Ke S, Ding X, Sun W, Gao J
American journal of translational research 2019;11(12):7555-7567

---

Trichomonas vaginalis Induces NLRP3 Inflammasome Activation and Pyroptotic Cell Death in Human Macrophages.

Riestra AM, Valderrama JA, Patras KA, Booth SD, Quek XY, Tsai CM, Nizet V
Journal of innate immunity 2019;11(1):86-98

---

TLR4/NF-κB Signaling Induces GSDMD-Related Pyroptosis in Tubular Cells in Diabetic Kidney Disease.

Wang Y, Zhu X, Yuan S, Wen S, Liu X, Wang C, Qu Z, Li J, Liu H, Sun L, Liu F
Frontiers in endocrinology 2019;10:603

---

Neferine inhibits LPS-ATP-induced endothelial cell pyroptosis via regulation of ROS/NLRP3/Caspase-1 signaling pathway.

Tang YS, Zhao YH, Zhong Y, Li XZ, Pu JX, Luo YC, Zhou QL
Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2019 Sep;68(9):727-738

---

Gasdermin D: Evidence of pyroptosis in spontaneous preterm labor with sterile intra-amniotic inflammation or intra-amniotic infection.

Gomez-Lopez N, Romero R, Tarca AL, Miller D, Panaitescu B, Schwenkel G, Gudicha DW, Hassan SS, Pacora P, Jung E, Hsu CD
American journal of reproductive immunology (New York, N.Y. : 1989) 2019 Dec;82(6):e13184

---

Endothelial pyroptosis underlies systemic inflammatory response following radiofrequency ablation of hepatic hemangiomas.

Wang S, Yang M, Yang X, Xu L, Ke S, Ding X, Sun W, Gao J
Scandinavian journal of clinical and laboratory investigation 2019 Dec;79(8):619-628

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Bacteroides fragilis Strain ZY-312 Defense against Cronobacter sakazakii-Induced Necrotizing Enterocolitis In Vitro and in a Neonatal Rat Model.

Fan H, Chen Z, Lin R, Liu Y, Wu X, Puthiyakunnon S, Wang Y, Zhu B, Zhang Q, Bai Y, Zhi F
mSystems 2019 Aug 6;4(4)

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Cytokine Secretion and Pyroptosis of Thyroid Follicular Cells Mediated by Enhanced NLRP3, NLRP1, NLRC4, and AIM2 Inflammasomes Are Associated With Autoimmune Thyroiditis.

Guo Q, Wu Y, Hou Y, Liu Y, Liu T, Zhang H, Fan C, Guan H, Li Y, Shan Z, Teng W
Frontiers in immunology 2018;9:1197

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Caspase-4 activation by a bacterial surface protein is mediated by cathepsin G in human gingival fibroblasts.

Jun HK, Jung YJ, Ji S, An SJ, Choi BK
Cell death and differentiation 2018 Feb;25(2):380-391

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Atypical Gasdermin D and Mixed Lineage Kinase Domain-like Protein Leakage Aggravates Tetrachlorobenzoquinone-Induced Nod-like Receptor Protein 3 Inflammasome Activation.

Xia X, Lu B, Dong W, Yang B, Wang Y, Qin Q, Liu Z, Song E, Song Y
Chemical research in toxicology 2018 Dec 17;31(12):1418-1425

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Protein kinase D at the Golgi controls NLRP3 inflammasome activation.

Zhang Z, Meszaros G, He WT, Xu Y, de Fatima Magliarelli H, Mailly L, Mihlan M, Liu Y, Puig Gámez M, Goginashvili A, Pasquier A, Bielska O, Neven B, Quartier P, Aebersold R, Baumert TF, Georgel P, Han J, Ricci R
The Journal of experimental medicine 2017 Sep 4;214(9):2671-2693

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Inflammasome activators induce fibronectin expression and release in macrophages.

Jun HK, Jung YJ, Choi BK
Cellular microbiology 2017 May;19(5)

---

Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores.

Liu X, Zhang Z, Ruan J, Pan Y, Magupalli VG, Wu H, Lieberman J
Nature 2016 Jul 7;535(7610):153-8

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Gasdermin D is an executor of pyroptosis and required for interleukin-1β secretion.

He WT, Wan H, Hu L, Chen P, Wang X, Huang Z, Yang ZH, Zhong CQ, Han J
Cell research 2015 Dec;25(12):1285-98

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 20770-1-AP(GSDMD antibody) at dilution of 1:300

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using GSDMD antibody 20770-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human skin using 20770-1-AP(GSDMD antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human skin using 20770-1-AP(GSDMD antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue