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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00079792-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00079792-M01, RRID:AB_426076
- Product name
- GSDMD monoclonal antibody (M01), clone 3F12-1B2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant GSDMD.
- Antigen sequence
MGSAFERVVRRVVQELDHGGEFIPVTSLQSSTGFQ
PYCLVVRKPSSSWFWKPRYKCVNLSIKDILEPDAA
EPDVQRGRSFHFYDAMDGQIQGSVELAAPGQAKIA
GGAAVSDSSSTSMNVYSLSVDPNTWQTLLHERHLR
QPEHKVLQQLRSRGDNVYVVTEVLQTQKEVEVTRT
HKREGSGRFSLPGATCLQGEGQGHLSQKKTVTIPS
GSTLAFRVAQLVIDSDLDVLLFPDKKQRTFQPPAT
GHKRSTSEGAWPQLPSGLSMMRCLHNFLTDGVPAE
GAFTEDFQGLRAEVETISKELELLDRELCQLLLEG
LEGVLRDQLALRALEEALEQGQSLGPVEPLDGPAG
AVLECLVLSSGMLVPELAIPVVYLLGALTMLSETQ
HKLLAEALESQTLLGPLELVGSLLEQSAPWQERST
MSLPPGLLGNSWGEGAPAWVLLDECGLELGEDTPH
VCWEPQAQGRMCALYASLALLSGLSQEPH- Isotype
- IgG
- Antibody clone number
- 3F12-1B2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- GSDMD monoclonal antibody (M01), clone 3F12-1B2 Western Blot analysis of GSDMD expression in Jurkat ( Cat # L017V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of GSDMD expression in transfected 293T cell line by GSDMD monoclonal antibody (M01), clone 3F12-1B2.Lane 1: GSDMD transfected lysate (Predicted MW: 52.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged GSDMDC1 is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to GSDMDC1 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to GSDMDC1 on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
