A02842

antibody from Boster Biological Technology

Targeting: GSDMD DF5L, FLJ12150, GSDMDC1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

A02842

Provider

Boster Biological Technology

Product name

Anti-GSDMD Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for GSDMD detection. Tested with WB, IHC-P, FCM, Direct ELISA in Human.

Reactivity

Human

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

In vitro and in silico analyses reveal the toxicity of metolachlor to grass carp hepatocytes and the antagonism of melatonin.

Chi Q, Xia Y, Luo D, Zhu L, Yang X, Li S
Pesticide biochemistry and physiology 2024 Jun;202:105930

---

Metformin inhibits EV71‑induced pyroptosis by upregulating DEP domain‑containing mTOR‑interacting protein.

Zhang X, Sun C, Hao J, Cao L, Zhang X, Du J, Han Q
Experimental and therapeutic medicine 2023 Aug;26(2):388

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of GSDMD using anti-GSDMD antibody (A02842). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human Jurkat whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSDMD antigen affinity purified polyclonal antibody (Catalog # A02842) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSDMD at approximately 53KD. The expected band size for GSDMD is at 53KD.

Additional image