Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00005184-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005184-M01, RRID:AB_2161520
- Product name
- Mouse Monoclonal PEPD Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PEPD - peptidase D
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Collagen-derived proline promotes pancreatic ductal adenocarcinoma cell survival under nutrient limited conditions.
Olivares O, Mayers JR, Gouirand V, Torrence ME, Gicquel T, Borge L, Lac S, Roques J, Lavaut MN, Berthezène P, Rubis M, Secq V, Garcia S, Moutardier V, Lombardo D, Iovanna JL, Tomasini R, Guillaumond F, Vander Heiden MG, Vasseur S
Nature communications 2017 Jul 7;8:16031
Nature communications 2017 Jul 7;8:16031
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PEPD Antibody (1D5-H3) [H00005184-M01] - Analysis of PEPD expression in transfected 293T cell line by PEPD monoclonal antibody (M01), clone 1D5-H3.Lane 1: PEPD transfected lysate (Predicted MW: 54.6 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PEPD Antibody (1D5-H3) [H00005184-M01] - PEPD monoclonal antibody (M01), clone 1D5-H3 Analysis of PEPD expression in HepG2.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: PEPD Antibody (1D5-H3) [H00005184-M01] - Detection limit for recombinant GST tagged PEPD is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: PEPD Antibody (1D5-H3) [H00005184-M01] - Analysis of monoclonal antibody to PEPD on HepG2 cell. Antibody concentration 10 ug/ml.