A05948

antibody from Boster Biological Technology

Targeting: AHSP EDRF, ERAF

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A05948

Provider

Boster Biological Technology

Product name

Anti-AHSP/Eraf Antibody

Antibody type

Polyclonal

Description

Polyclonal antibody for ERAF/AHSP detection. Host: Rabbit.Size: 100μL. Tested applications: IHC. Reactive species: Human. ERAF/AHSP information: Molecular Weight: 11840 MW; Subcellular Localization: Cytoplasm ; Tissue Specificity: Expressed in blood and bone marrow.

Reactivity

Human

Host

Rabbit

Vial size

100μL

Concentration

86 mg/mL by Refractometry

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemistry of Rabbit anti-AHSP antibody. AHSP and CD71 stain megakaryocytes in primary myelofibrosis. Tissue: A.) Primary myelofibrosis, H&E. B.) CD235a stains both nucleated EPs and mature, anucleate RBCs. AHSP C.) and CD71. D.) variably stain megakaryocytes and also stain nucleated EPs. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin. Antigen retrieval: TRIS-EDTA pH9.0. Primary antibody: AHSP antibody at 1:8,000 for overnight at 4°C Secondary antibody: anti-rabbit secondary at 1:10,000 for 45 min at RT. Localization: Anti-AHSP is cytoplasmic. Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain.

Additional image