AMAb91089

antibody from Atlas Antibodies

Targeting: DDC AADC

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

AMAb91089

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#AMAb91089, RRID:AB_2665795

Product name

Anti-DDC

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

LETVMMDWLGKMLELPKAFLNEKAGEGGGVIQGSA
SEATLVALLAARTKVIHRLQAASPELTQAAIMEKL
VAYSSDQAHSSVERAGLIGGVKLKAIPSDGNFAMR
ASA


Epitope

Binds to an epitope located within the peptide sequence MDWLGKMLEL as determined by overlapping synthetic peptides.

Isotype

IgG

Antibody clone number

CL2962

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Kidney tissue

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human kidney and fallopian tube tissues using AMAb91089 antibody. Corresponding DDC RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Show more

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of rat substantia nigra shows strong immunoreactivity in dopamine neurons.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of mouse substantia nigra shows strong positivity in dopamine neurons.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human substantia nigra shows strong immunoreactivity in dopamine neurons.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of rat substantia nigra shows strong positivity in dopamine neurons in pars compacta.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of mouse substantia nigra shows strong positivity in dopamine neurons in pars compacta.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human substantia nigra shows cytoplasmic positivity in dopamine neurons in pars compacta.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human fallopian tube shows no positivity in glandular cells as expected.