H00001644-M18

antibody from Abnova Corporation

Targeting: DDC AADC

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001644-M18

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001644-M18, RRID:AB_10961898

Product name

DDC monoclonal antibody (M18), clone 8E8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant DDC.

Antigen sequence

MNASEFRRRGKEMVDYVANYMEGIEGRQVYPDVEP
GYLRPLIPAAAPQEPDTFEDIINDVEKIIMPGVTH
WHSPYFFAYFPTASSYPAMLADMLCGAIGCIGFSW
AASPACTELETVMMDWLGKMLELPKAFLNEKAGEG
GGVIQGSASEATLVALLAARTKVIHRLQAASPELT
QAAIMEKLVAYSSDQAHSSVERAGLIGGVKLKAIP
SDGNFAMRASALQEALERDKAAGLIPFFMVATLGT
TTCCSFDNLLEVGPICNKEDIWLHVDAAYAGSAFI
CPEFRHLLNGVEFADSFNFNPHKWLLVNFDCSAMW
VKKRTDLTGAFRLDPTYLKHSHQDSGLITDYRHWQ
IPLGRRFRSLKMWFVFRMYGVKGLQAYIRKHVQLS
HEFESLVRQDPRFEICVEVILGLVCFRLKGSNKVN
EALLQRINSAKKIHLVPCHLRDKFVLRFAICSRTV
ESAHVQRAWEHIKELAADVLRAERE


Isotype

IgG

Antibody clone number

8E8

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of DDC expression in transfected 293T cell line by DDC monoclonal antibody (M18), clone 8E8.Lane 1: DDC transfected lysate (Predicted MW: 53.9 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged DDC is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol