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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-21533 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IFI30 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Raji. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references A connexin/ifi30 pathway bridges HSCs with their niche to dampen oxidative stress.
Cacialli P, Mahony CB, Petzold T, Bordignon P, Rougemont AL, Bertrand JY
Nature communications 2021 Jul 23;12(1):4484
Nature communications 2021 Jul 23;12(1):4484
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using IFI30 Polyclonal Antibody (Product # PA5-21533). Sample (30 µg whole cell lysate). A: Raji. 12% SDS PAGE. IFI30 Polyclonal Antibody (Product # PA5-21533) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IFI30 Polyclonal Antibody detects IFI30 protein at cytosol on AGS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded AGS xenograft. IFI30 Polyclonal Antibody (Product # PA5-21533) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 7 HSPCs interact with IFI30 + cells in human fetal liver. a Double immunostaining against GILT/IFI30 and CD117 on sections of human fetal liver at different stages of gestation. b Distribution of GILT/IFI30 + cells (red line), CD117 + cells (brown line), and IFI30 + cells in contact with CD117 + cells (green line). For each developmental stage, only one human fetal liver sample was used ( N = 1), but three different sections were analyzed. c High magnification of double immunostaining against GILT/IFI30 (red) CD117 (brown) and nuclei (blue), on sections of human fetal liver at 13 weeks of gestation. Scale bars: 10 mum. d Double immunostaining against GILT/IFI30 (black), CD68 (cyan), and nuclei (blue) in sections of human fetal liver at 13 weeks of gestation. All red arrows point to double-stained cells. Scale bars: 100 mum ( a ); 25 mum ( c ); 200 mum. Right panel shows a magnification of the region delimited by the red dotted line ( d ).
