GTX48704

antibody from GeneTex

Targeting: CASZ1 castor, cst, FLJ20321, SRG, ZNF693

ELISA

Antibody data

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Validation data

CASZ1 protein structure - GTX48704 shown in red.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Western blot using GeneTex's anti-hCASZ1 antibody. This blot shows detection of endogenous and transfected human CASZ1 protein in fresh whole cell lysate (~30 µg). Protein was resolved by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000, incubated 1.5 hours at room temperature, and incubated with HRP-conjugated Goat Anti-Rabbit antibody for 45 min. at room temperature. Lane 1, BE2(s) cell lysate. Lane 2, BE2(N) cell lysate. Lane 3, SY5Y transfected with hCASZ5 (125kDa). Lane 4, SY5Y transfected with hCASZ11 (190kDa). Personal communication, Carol Thiele, NCI, Bethesda, MD.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Western blot using GeneTex's anti-hCASZ1 antibody. This blot shows detection of endogenous and transfected human CASZ1 protein in fresh whole cell lysate (~30 ?g). Protein was resolved by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000, incubated 1.5 hours at room temperature, and incubated with HRP-conjugated Goat Anti-Rabbit antibody for 45 min. at room temperature. Lane 1, BE2(s) cell lysate. Lane 2, BE2(N) cell lysate. Lane 3, SY5Y transfected with hCASZ5 (125kDa). Lane 4, SY5Y transfected with hCASZ11 (190kDa).

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Western Blot of Anti-CASZ1 Antibody. Lane 1: NBLS Cytoplasmic (20?g). Lane 2: NBLS Nuclear (3?g). Lane 3: BE2C Cytoplasmic (30?g). Lane 4: BE2C Nuclear (7?g). Lane 5: SY5Y-CASZ1b (10?g). Block: 5% Blotto/TTBS for 1 hour. Primary: Casz1 1:10,000 for 1 hour. Secondary: Goat anti-Rabbit HRP for 1 hour. 240sec exposure. Detects nuclear endogenous CASZ1a and CASZ1b; and transiently transfected CASZ1b isoform.