AP09422PU-S

antibody from OriGene

Targeting: CASZ1 castor, cst, FLJ20321, SRG, ZNF693

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AP09422PU-S

Provider

OriGene

Product name

Rabbit Polyclonal Anti-CASZ1 Antibody, Aff - Purified

Antibody type

Polyclonal

Description

Rabbit Polyclonal Anti-CASZ1 Antibody, Aff - Purified

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

25ul

Concentration

1.09 mg/mL by UV absorbance at 280 nm

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunoblot using anti-hCASZ1 antibody. This blot shows detection of endogenous and transfected human CASZ1 protein in fresh whole cell lysate (~30 ug). Protein was resolved by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000, incubated 1.5 hours at room temperature, and incubated with HRPconjugated Goat Anti-Rabbit antibody for 45 min. at room temperature. Lane 1, BE2(s) cell lysate. Lane 2, BE2(N) cell lysate. Lane 3, SY5Y transfected with hCASZ5 (125kDa). Lane 4, SY5Y transfected with hCASZ11 (190kDa).

Validation comment

WB