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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- GTX85729 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX85729, RRID:AB_10722554
- Product name
- CASZ1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot of affinity purified anti-hCas5 antibody shows detection of a band ~125 kD in size corresponding to hCas5 (arrowhead). Lane 1 contains a lysate from AS cells transfected with empty vector Lane 2 contains lysate from AS cells transfected with hCas5 (predicted MW 125 kD) Lane 3 contains lysate from BE2 cells that have been shown to contain a high level of hCas mRNA (as tested by Northern blot) Lane 4 contains lysate from human muscle tissue that also shows high levels of hCas mRNA Specific band reactivity is effectively competed when the antibody is pre-incubated with the immunizing peptide (data not shown)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Human Lung (formalin-fixed, paraffin-embedded) stained with CASZ1 antibody (GTX85729) at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.
