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LearnNB100-74614
antibody from Novus Biologicals
Targeting: CSTF2T
CstF-64T, DKFZp434C1013, KIAA0689, tauCstF-64
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- NB100-74614 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-74614, RRID:AB_1048622
- Product name
- Rabbit Polyclonal CSTF2T Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CSTF2T Antibody [NB100-74614] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-74614 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. CSTF2T/TauCSTF64 was also immunoprecipitated by rabbit anti-CSTF2T/TauCSTF64 antibody NB100-74613, which recognizes an upstream epitope.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CSTF2T Antibody [NB100-74614] - Detection of Human CSTF2T by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug), and Jurkat (50ug) cells. Antibodies: Affinity purified rabbit anti-CSTF2T antibody NB100-74614 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: CSTF2T Antibody [NB100-74614] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-CSTF2T/TauCSTF64 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CSTF2T Antibody [NB100-74614] - FFPE section of human skin squamous cell carcinoma. NB100-74614 used at a dilution of 1:250. DAB staining using Immunohistochemistry Accessory Kit
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CSTF2T Antibody [NB100-74614] - Human skin basal cell carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).
