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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-47865 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PCDH15 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Protocadherin-1, rhProtocadherin-8, and rhProtocadherin-10 is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of HEK293T human embryonic kidney cell line and HEK293T cell line spiked with recombinant human Protocadherin-15 (5 ng/lane). PVDF membrane was probed with 2 µg/mL of Sheep Anti-human Protocadherin-15 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47865) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for Protocadherin-15 at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PCDH15 in 5 ng/lane HEK293T human embryonic kidney cell line and HEK293T cell line spiked with recombinant Human Protocadherin-15. Samples were incubated in PCDH15 polyclonal antibody (Product # PA5-47865) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for Protocadherin-15 at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of PCDH15 in immersion fixed YT human leukemia natural killer-like cell line. Samples were incubated in PCDH15 polyclonal antibody (Product # PA5-47865) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of PCDH15 in immersion fixed YT human leukemia natural killer-like cell line. Samples were incubated in PCDH15 polyclonal antibody (Product # PA5-47865) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Protocadherin-15 was detected in immersion fixed YT human leukemia natural killer-like cell line using Sheep Anti-human Protocadherin-15 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47865) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-Sheep IgG Secondary Antibody (re and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PCDH15 in immersion fixed paraffin-embedded sections of human kidney. Samples were incubated in PCDH15 polyclonal antibody (Product # PA5-47865) using a dilution of 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules.
