11003-1-AP

antibody from Invitrogen Antibodies

Targeting: TCOF1 TCS, treacle

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

11003-1-AP

Provider

Invitrogen Antibodies

Product name

TCOF1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAEARKRRE LLPLIYHHLL RAGYVRAARE VKEQSGQKCF LAQPVTLLDI YTHWQQTSEL GRKRKAEEDA ALQAKKTRVS DPISTSESSE EEEEAEAETA KATPRLASTN SSVLGADLPS SMKEKAKAET EKAGKTGNSM PHPATGKTVA NLLSGKSPRK SAEPSANTTL VSETEEEGSV PAFGAAAKPG MVSAGQADSS SEDTSSSSDE TDVEGKPSVK PAQVKASSVS TKESPARKAA PAPGKVGDVT PQVKGGALPP AKRAKKPEEE SESSEEGSES EEEAPAGTRS QVKASEKILQ VRAASAPAKG TPGKGATPAP PGKAGAVASQ TKAGKPEEDS ESSSEESSDS E (1-350 aa encoded by BC014559)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.3 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 11003-1-AP (TCOF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 11003-1-AP (TCOF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of TCOF1 antibody (11003-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TCOF1 transfected Jurkat cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neuro-2a cells were subjected to SDS PAGE followed by western blot with 11003-1-AP (TCOF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 11003-1-AP (TCOF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using TCOF1 antibody 11003-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IGG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lymphoma using 11003-1-AP (TCOF1 antibody) at dilution of 1:50 (under 10x lens).