20175-1-AP

antibody from Invitrogen Antibodies

Targeting: NCOA5 bA465L10.6, CIA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

20175-1-AP

Provider

Invitrogen Antibodies

Product name

NCOA5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: DMRDSRDPM YRREGSYDRY LRMDDYCRRK DDSYFDRYRD SFDGRGPPGP ESQSRAKERL KREERRREEL YRQYFEEIQR RFDAERPVDC SVIVVNKQTK DYAESVGRKV RDLGMVVDLI FLNTEVSLSQ ALEDVSRGGS PFAIVITQQH QIHRSCTVNI MFGTPQEHRN MPQADAMVLV ARNYERYKNE CREKEREEIA RQAAKMADEA ILQERERGGP EEGVRGGHPP AIQSLINLLA DNRYLTAEET DKIINYLRER KERLMRSSTD SLPG (112-384 aa encoded by B C056872)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.31 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 20175-1-AP (NCOA5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 20175-1-AP (NCOA5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using NCOA5 antibody 20175-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HEK-293 cells were stained with 0.2ug NCOA5 antibody (20175-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-NCOA5 (IP:20175-1-AP, 4ug; Detection:20175-1-AP 1:800) with HEK-293 cells lysate 3000ug.