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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00053358-D01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00053358-D01, RRID:AB_10713966
- Product name
- SHC3 MaxPab rabbit polyclonal antibody (D01)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human SHC3 protein.
- Antigen sequence
MLPRTKYNRFRNDSVTSVDDLLHSLSVSGGGGKVS
AARATPAAAPYLVSGEALRKAPVDGPGSLGHLLHK
VSHLKLSSSGLRGLSSAARERAGARLSGSCSAPSL
AAPDGSAPSAPRAPAMSAARKGRPGDEPLPRPPRG
APHASDQVLGPGVTYVVKYLGCIEVLRSMRSLDFS
TRTQITREAISRVCEAVPGAKGAFKKRKPPSKMLS
SILGKSNLQFAGMSISLTISTASLNLRTPDSKQII
ANHHMRSISFASGGDPDTTDYVAYVTKDPVNRRAC
HILECCDGLAQDVIGSIGQAFELRFKQYLQCPTKI
PALHDRMQSLDEPWTEEEGDGSDHPYYNSIPSKMP
PPGGFLDTRLKPRPHAPDTAQFAGKEQTYYQGRHL
GDTFGEDWQQTPLRQGSSDIYSTPEGKLHVAPTGE
APTYVNTQQIPPQAWPAAVSSAESSPRKDLFDMKP
FEDALKNQPLGPVLSKAASVECISPVSPRAPDAKM
LEELQAETWYQGEMSRKEAEGLLEKDGDFLVRKST
TNPGSFVLTGMHNGQAKHLLLVDPEGTIQTKDRVF
DSISHLINHHLESSLPIVSAGSELCLQQPVERKQ- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of SHC3 expression in transfected 293T cell line (H00053358-T01) by SHC3 MaxPab polyclonal antibody.Lane 1: SHC3 transfected lysate(64.00 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of SHC3 transfected lysate using anti-SHC3 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with SHC3 purified MaxPab mouse polyclonal antibody (B01P) (H00053358-B01P).
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
