Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP2-15163 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Wnt-7b Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Increased glycolysis mediates Wnt7b-induced bone formation.
Chen H, Ji X, Lee WC, Shi Y, Li B, Abel ED, Jiang D, Huang W, Long F
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Jul;33(7):7810-7821
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Jul;33(7):7810-7821
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Wnt7b Antibody [NBP2-15163] - Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Wnt7b Antibody [NBP2-15163] - Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: WNT7B protein transfected 293T lysates 10% SDS PAGE; antibody diluted at 1:5000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Wnt-7b Antibody [NBP2-15163] - Analysis. Mouse tissue extracts (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt7b antibody at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Wnt-7b Antibody [NBP2-15163] - Non-transfected (-) and Wnt7b-transfected (+, including V5-tag) 293T whole cell extracts (72 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt7b antibody at a dilution of 1:5000. The V5 was used as positive control (1:2500) shown at the bottom panel. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Wnt-7b Antibody [NBP2-15163] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt7b antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Wnt7b Antibody [NBP2-15163] - Paraffin-embedded Ovarian carcinoma, using antibody at 1:250 dilution.