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- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-24796 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Dopamine Transporter Monoclonal Antibody (mAb16)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- mAb16
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Long-term RNAi knockdown of α-synuclein in the adult rat substantia nigra without neurodegeneration.
Zharikov A, Bai Q, De Miranda BR, Van Laar A, Greenamyre JT, Burton EA
Neurobiology of disease 2019 May;125:146-153
Neurobiology of disease 2019 May;125:146-153
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Dopamine Transporter in rat striatal membrane tissue lysate. Sample was incubated in Dopamine Transporter monoclonal antibody (Product # MA5-24796).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Dopamine Transporter in rat striatal membrane tissue lysate. Sample was incubated in Dopamine Transporter monoclonal antibody (Product # MA5-24796).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using anti-Dopamine Transporter Monoclonal Antibody (mAb16) (Product # MA5-24796). A 68 kDa band corresponding to Dopamine Transporter (DAT) was observed across cell lines and brain tissues except Mouse, Rat Cerebellum and Mouse, Rat Heart which are reported to be low to negative expression models for Dopamine Transporter. Another band at 75 kDa was found only in the cell lines which could be another isoform of DAT. Uncharacterized bands at 260, 35, 15 kDa were observed in the samples tested. A band at 25 kDa was observed in mouse tissues which corresponds to circulating IgG in tissues. Membrane enriched extracts (30 µg) of BeWo (Lane1), MCF7 (Lane 2), SH-SY5Y (Lane 3), tissue extracts of Mouse Brain (Lane 4), Rat Brain (Lane 5), Mouse Cerebellum (Lane 6), Rat Cerebellum (Lane 7), Mouse Heart (Lane 8) and Rat Heart (Lane 9) were electrophoresed using Novex® NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SLC6A3/DAT1 in PC12 cells using SLC6A3/DAT1 monoclonal antibody (Product # MA5-24796) with a dilution of 1:250, Phalloidin Alexa Fluor 568 (Red) and DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Dopamine Transporter in mouse brain. Samples were incubated in Dopamine Transporter monoclonal antibody (Product # MA5-24796) followed by DAB with hematoxylin counterstain.
